Photobiomodulation in human neuroblastoma under oxidative stress

Background With the increase in life expectancy, number of people with neurodegenerative diseases such as Alzheimer’s Disease (AD) is rising rapidly. Considering pathways AD, one its fundamental processes oxidative stress, affecting central nervous system and cell regeneration. Interestingly, last decades, photobiomodulation has shown positive effects on neural cells that are compromised by AD. Studies have demonstrated LED (light emitting diode) irradiation regulates function, stimulating proliferation, promoting biomodulation cytoskeleton, reducing apoptosis. To contribute elucidation importance this study aims to evaluate neuroprotective effect, comparing different energy density irradiating SH-SY5Y (human neuroblastoma) under effect stress induced hydrogen peroxide (H2O2). Method were cultivated DMEM/F12 medium (Dulbecco’s modified Eagles - Gibco), supplemented 10% fetal bovine serum 1% antibiotic antimycotic, at 37°C, an atmosphere 5% CO2. Cells plated 24-well plates. After adhesion, irradiated 660 nm (LED Biopdi/Irrad-Led5), densities 1 J/cm2, 3 5 J/cm2 10 J/ cm2. 24 h after irradiation, was 200 μM H2O2 for 24h (this parameter had been previously determined ROSSATO, R. C. et al, 2021). Result Once stressed, a considerable statistic difference observed all evaluated, compared stressed-only group Importantly, lowest obtained better statistical mithocondrial activity (p < 0.0001). Conclusion It possible determine compare promote cellular photobiomodulation, inducing neubroblastoma. vitro model AD approaching novel concepts preventive tool. al. Neuroprotective taurine hydrocortisone stress. Research, Society Development, v. 10, n. 9, p. e55510918426-e55510918426, 2021